What is the difference between kanamycin A and kanamycin B?
Kanamycin A has a hydroxy group at position C2′, whereas kanamycin B possesses an amino group at this position (Fig 1B). Their specific interactions with bacterial ribosomal RNAs to inhibit bacterial protein synthesis have been clearly observed through X-ray structural analysis [2].
How do you dilute kanamycin?
You can dilute 25 mg of kanamycin and dilute with 1 mL of dH2O and finally filter sterilized. Keep your kanamycin stock in -20C/-30C for long term use. Take 20 ul of your kanamycin stock and add with 9980 ul of sterilized dH2O.
How long is kanamycin stable at 4c?
Stable for for 12 months at 4°C. Further dilutions can be made in assay buffer. Stability at 37°C: 5 days.
How much does kanamycin cost?
Kanamycin sulfate
| Pricing | Availability |
|---|---|
| 1 g / $47 | Ships in 5-7 Business Days |
What is the generic name of kanamycin?
Kantrex (kanamycin) Injection is an aminoglycoside antibiotic used to treat serious infections caused by bacteria.
What is the drug classification for kanamycin?
Kanamycin belongs to the class of medicines known as aminoglycoside antibiotics. It works by killing bacteria or preventing their growth. However, this medicine will not work for colds, flu, or other virus infections.
How much kanamycin do you put on a LB plate?
Autoclave 1 L of LB agar. Cool to 55°C. Add 1 mL of the kanamycin stock (i.e., 50 mg).
Is kanamycin soluble in water?
Kanamycin sulfate is soluble in water (50 mg/ml), yielding a clear solution. Kanamycin sulfate is practically insoluble in alcohol, acetone, chloroform, ether, and ethyl acetate. A 1% solution in water has a pH of 6.5 to 8.5.
How long can you keep kanamycin plates?
Kanamycin sulfate can be added into agar (agar plate), and the plate should be then be sealed to prevent any evaporation of moisture from the agar. The sealed plate can be stored at 2-8 °C for about one month. If the plate is not sealed to prevent evaporation of moisture, the kanamycin can degrade.
How long is kanamycin stable in media?
Kanamycin is light sensitive. Keep tightly closed in light-resistant containers. “Solutions are stable at 37°C for approximately 5 days. Aqueous stock solutions can be stored at 2-8°C for long term storage”, Sigma.
Is kanamycin still used?
Kanamycin injection is used to treat serious bacterial infections in many different parts of the body. This medicine is for short-term use only (usually 7 to 10 days). Kanamycin belongs to the class of medicines known as aminoglycoside antibiotics.
What is kanamycin used for?
Kanamycin injection is used to treat serious bacterial infections in many different parts of the body. This medicine is for short-term use only (usually 7 to 10 days). Kanamycin belongs to the class of medicines known as aminoglycoside antibiotics. It works by killing bacteria or preventing their growth.
What infection does kanamycin treat?
Kanamycin may be considered as initial therapy in the treatment of infections where one or more of the following are the known or suspected pathogens: E. coli, Proteus species (both indole-positive and indole-negative), Enterobacter aerogenes, Klebsiella pneumoniae, Serratia marcescens, Acinetobacter species.
Is kanamycin a broad-spectrum antibiotic?
Kanamycin A is similar to streptomycin and neomycines, and it possesses a broad spectrum of antimicrobial action. It is active with respect to most Gram-positive and Gram-negative microorganisms (staphylococci, colon bacillus, klebisella, Fridlender’s bacillus, proteus, shigella, salmonella).
How do I make kanamycin 1000x stock?
Directions:
- Dissolve 0.5 g of kanamycin into 10 ml of ddH2O.
- Filter through a 0.22 µm filter to sterilize.
- Aliquot and store at -20°C.
- Use at 1:1000 dilution in LB or LB-Agar.
How long are LB kanamycin plates good for?
The sealed plate can be stored at 2-8 °C for about one month. If the plate is not sealed to prevent evaporation of moisture, the kanamycin can degrade. For Laboratory Use Only.
What is kanamycin used for in lab?
Kanamycin acts by binding to the 30S subunit of the bacterial ribosome and inhibiting protein synthesis in susceptible bacteria. Gibco™ Kanamycin is effective against a wide variety of gram-negative and some gram-positive bacteria, and is used for the prevention of bacterial contamination of cell cultures.
Can kanamycin be stored at room temperature?
Shipping and Storage: Kanamycin Sulfate is shipped at room temperature. For maximum stability and long-term use, store at room temperature upon receipt. Protect from moisture. Antibiotic solutions are usually added to freshly autoclaved media (after they have cooled to about 50°C).
How do you store kanamycin?
At what temperature should kanamycin be stored?
Storage/Stability Solutions are stable at 37 °C for approximately 5 days. Aqueous stock solutions can be stored at 2-8 °C for long term storage.