What is metabolite analysis?
Metabolomics is a field of research that investigates the complex and diverse metabolic processes in biological systems. The goal of metabolome analysis is to identify and quantify all metabolites in a cellular system.
How are metabolites measured?
The most commonly used platforms for the detection and measurement of metabolites involve their separation by gas chromatography (GC), liquid chromatography (LC), or capillary electrophoresis (CE) coupled with subsequent mass spectrometry (MS) of the separated molecules (Figs 3.1 and 3.2).
What is metabolite in microbiology?
In biochemistry, a metabolite is an intermediate or end product of metabolism. The term metabolite is usually used for small molecules.
What is an example of a metabolite?
Examples of primary metabolites are ethanol, glutamic acid, aspartic acid, 5′ guanylic acid, acetic acid, lactic acid, glycerol, etc. Examples of secondary metabolites are pigments, resins, terpenes, ergot, alkaloids, antibiotics, naphthalenes, nucleosides, quinolones, peptides, growth hormones, etc.
What is the role of metabolites?
Metabolites are the products and intermediates of cellular metabolism. Metabolites can have a multitude of functions, including energy conversion, signaling, epigenetic influence, and cofactor activity (Lu and Thompson, 2012; Wellen and Thompson, 2012).
What is metabolomics in genomics?
Metabolomics is the quantification of small molecules produced by metabolic processes within a biological sample. Metabolomics datasets contain a wealth of information that reflect the disease state and are consequent to both genetic variation and environment.
What is a metabolomic profile?
Metabolomic profiling is a technique that provides a snapshot of an organism’s physiological state at a given point in time12. Changes in metabolomic profiles can be a result of altered gene expression13,14, but may also be due to post-transcriptional processes12.
Why do we use metabolomics?
Metabolomics is an objective lens to view the complex nature of how physiology is linked to external events and conditions, as well as measure its response to perturbations such as those associated with disease.