What is electrokinetic effect?
Electrokinetic effects are associated with the relationship between the relative motion of two phases (generally a liquid and a solid) and the electrical properties of the interface between them. Electrokinetic phenomena arise in microheterogeneous systems, i.e. in cases when one phase is dispersed in another.
What is electrokinetic phenomena in colloids?
Electrophoresis, which is one of the electrokinetic phenomena observed in colloidal systems, is the motion of charged colloidal particles in a liquid medium under an applied electric field.
Which of the following is electrokinetic phenomena?
The four main electrokinetic phenomena are electrophoresis, electroosmosis, streaming potential, and sedimentation potential, or Dorn effect. These phenomena are related to one another through the zeta potential ζ of the electrical double layer that exists in the neighborhood of the charged surface.
What is electrokinetic injection?
The electrokinetic injection is the most common method to inject a sample solution in microchip-based CE. The electrokinetic injection method is based on the electroosmotic flow (EOF) generated by applying high voltages in a microchannel. In electrokinetic injection, tee [5. A.
How does zeta potential work?
Zeta potential is a physical property which is exhibited by any particle in suspension, macromolecule or material surface. It can be used to optimize the formulations of suspensions, emulsions and protein solutions, predict interactions with surfaces, and optimise the formation of films and coatings.
What is electrokinetic injection capillary electrophoresis?
In electrokinetic injection, the amount injected of a certain sample component depends on the component’s mobility, the capillary diameter, the applied electric field and the injection time. This means that sample components with different mobilities are injected in different amounts .
What are the advantages of hydrodynamic injection over electrokinetic introduction?
The advantage of hydrodynamic injection is that the quan- tity of individual analytes injected is constant, independent of their own mobility, in contrast to electrokinetic injection. This mode is mechanically the simplest one and adequate for samples with a low viscosity.
What is zeta potential give examples?
The influence of known contaminants on the zeta potential of a sample can be a powerful tool in formulating the product to resist flocculation for example. When an electric field is applied across an electrolyte, charged particles suspended in the electrolyte are attracted towards the electrode of opposite charge.
What is zeta or electrokinetic potential?
Zeta potential is an electrokinetic potential at the shear plane (the boundary between the compact layer and the diffuse layer) near a solid-liquid interface where the liquid velocity is zero.
What is hydrodynamic injection?
The hydrodynamic tail vein injection is a technique that is used to deliver nucleic acids into live mice. Delivery through this method results in the in vivo transfection of foreign DNA primarily in the liver.
What zeta potential tells us?
The zeta potential is an important and readily measurable indicator of the stability of colloidal dispersions. The magnitude of the zeta potential indicates the degree of electrostatic repulsion between adjacent, similarly charged particles in a dispersion.
What is zeta potential give an example?
What is zeta potential in simple words?
The zeta potential is a measure of the difference in potential between the bulk fluid in which a particle is dispersed and the layer of fluid containing the oppositely charged ions that is associated with the nanoparticle surface.
What is hydrodynamic gene delivery?
Hydrodynamic gene delivery uses a hydrodynamic force generated by a pressurized injection of a large volume of DNA solution into the blood vessel so as to permeabilize the capillary endothelium and generate “pores” in the plasma membrane of the surrounding parenchyma cells, through which DNA or other macromolecules of …
How does hydrodynamic sample injection work in capillary electrophoresis?
Hydrodynamic injection is accomplished by the application of a pressure difference between the two ends of a capillary. The amount of sample injected can be calculated by the Poiseuille equation.
Why is zeta potential an important indicator?
Almost all particulate or macroscopic materials in contact with a liquid acquire an electronic charge on their surfaces. Zeta potential is an important and useful indicator of this charge which can be used to predict and control the stability of colloidal suspensions or emulsions.
How does hydrodynamic injection work?
Why does pH affect zeta potential?
pH is the most important parameter for zeta potential. For example, if we added acid in nanofluid, pH will decrease, which will increase positive charges on the particle surface. Zeta potential will increase. The point at which zero electrophoretic mobility occurs is called an isoelectric point [34].