What is FaSSIF and FeSSIF?
FaSSIF, FeSSIF and FaSSGF are dissolution media that simulate human gut fluids: FaSSIF: Fasted State Simulated Intestinal Fluid. FeSSIF: Fed State Simulated Intestinal Fluid. FaSSGF: Fasted State Simulated Gastric Fluid.
What does FaSSIF mean?
Fasted State Simulated Intestinal Fluid
FaSSIF (Fasted State Simulated Intestinal Fluid) is a very useful drug development tool. Tests in this dissolution medium can reveal how your oral drug is likely to dissolve and potentially be absorbed in fluid from the upper intestine after drinking a glass of water.
What is FaSSIF solubility?
In FaSSIF, a more than 30-fold increase in apparent solubility as compared to phosphate buffer is seen. Apparent solubility accounts for both, moleculary dissolved API and micellarly solubilized drug. In addition, the concentration of molecularly dissolved API was evaluated separately by inverse dialysis.
What is FaSSIF pH?
Fasted State Simulated Intestinal Fluid (FaSSIF) Media has a pH of 6.50 and an osmolality of about 270 mOsmol/kg.
Why is simulated gastric fluid used?
Often for science projects, you may need to make simulated stomach acid. This can help you understand how different foods and certain drugs for stomach trouble will react with the acid in the stomach. 1. Simulated Gastric Fluid is a sterile solution and can be used directly.
What is simulated gastric fluid?
Simulated Gastric Fluid This medium contains hydrochloric acid and sodium chloride, as well as pepsin and water, and has a pH of 1.2. Although the medium addresses many of the qualities of gastric juice, there are some aspects that could be optimized.
What is Biorelevant media in dissolution?
Biorelevant media simulate gut fluids more accurately than any other dissolution media. They contain components (bile salts, phospholipids and salts) which replicate conditions found in the gastrointestinal tract such as the solubilizing properties, pH and osmolality.
How do you prepare simulated intestinal fluid?
The simulated intestinal fluid was prepared by the similar method: potassium phosphate monobasic (10.2 g) and SDS (3.75 g) were dissolved in a same 1000 ml of deionized water and then pH adjusted to 6.8 ± 0.1 with 1 N NaOH. Finally, the volume of each fluid was adjusted to 1500 (ml) with deionized water.
How do you make simulated gastric fluid?
The simulated gastric fluid was prepared by dissolving NaCl (3 g) in about 1450 (ml) of deionized water and then adjusted pH to 1.2 ± 0.1 with diluted HCl.
What is the pH of simulated intestinal fluid?
The traditional medium to simulate gastric conditions in the fasted state has been simulated gastric fluid (SGF) of the USP. This medium contains hydrochloric acid and sodium chloride, as well as pepsin and water, and has a pH of 1.2.
What is artificial gastric juice?
Artificial gastric fluid (Simulated Gastric Fluid) is a solution that simulates the composition and pH of gastric juice. The reagent is sterilized by 0.2 μm filtration. Artificial gastric juice is mainly composed of sodium chloride, dilute acid, pepsin, etc.
How do you get simulated intestinal fluid?
What is f2 value in dissolution?
An average difference of 10% at all measured time points results in a f2 value of 50. FDA has set a public standard of f2 value between 50-100 to indicate similarity between two dissolution profiles.
What is f2 in dissolution?
The factor f2, known as the similarity factor, measures the closeness between the two profiles:(1) f 2 = 50 · log 1 + 1 n ∑ t = 1 n ( R t – T t ) 2 – 0.5 × 100 where n is the number of time points, Rt and Tt are the dissolution value of the reference and test product at time t, respectively.