What are 293T cells?

The 293T cell line, originally referred as 293tsA1609neo, is a highly transfectable derivative of human embryonic kidney 293 cells, and contains the SV40 T-antigen. Age fetus Comments. This cell line is competent to replicate vectors carrying the SV40 region of replication.

What is the difference between 293 and 293T cells?

The 293T cells are derived from 293 cells but stably express the SV40 large T antigen which can bind to SV40 enhancers of expression vectors to increase protein production. 293T are also neomycin resistant due to the presence of a neomycin resistance cassete together with the SV40 Large T .

What are HEK293T 17 cells?

293T/17 [HEK 293T/17] is a cell line exhibiting epithelial morphology that was isolated from human embryo kidney tissue. The cell line is a derivative of the 293T (293tsA1609neo) cell line, a highly transfectable derivative of the 293 cell line into which the temperature sensitive gene for SV40 T-antigen was inserted.

Are HEK293T cells cancerous?

Thus, HEK293 is not a model for normal human cells; these cells are immortalized already by known oncogene but not malignant yet.

How do 293T cells grow?

Place the cells in the 37 °C incubator with 5% CO2. 6. Gently replace medium after 24 hours with 14 mL HEK293T Culture Medium and continue to culture at 37 ºC with 5% CO2. Cells should be ready for passage or expansion to a T175 flask in ~ 2-3 days.

Why do viruses have 293T cells?

The reason for the preference of HEK293T cells is that the presence of the SV40 T-antigen in the producer cells renders them more efficient for vector production. In addition, HEK293T cells show increased cell growth and transfection efficiency in comparison to HEK293 cells.

How do 293t cells grow?

Are 293T cells immortal?

Human embryonic kidney 293 cells, also often referred to as HEK 293, HEK-293, 293 cells, or less precisely as HEK cells, are a specific immortalised cell line derived from a spontaneously miscarried or aborted fetus or human embryonic kidney cells grown in tissue culture taken from a female fetus in 1973.

How long does it take for 293T cells to adhere?

Most HEK-293T cells double every 12–20 hours depending on conditions. Our cell doubling time is typically closer to 12 hours.

How do you maintain a 293T cell?

Cell maintenance Fresh HEK293T Culture Medium should be added to the cells every 3 days or as required by the growth rate of the cells. 2. HEK293T cells should always be treated very gently as they detach easily from the plate.

Why are 293t cells used for transfection?

Advantages of HEK293 cells Firstly, they are very easy to grow and to maintain, with high reproducibility, which makes them preferable over other less-robust and slow-growing cell lines. Furthermore, they are very efficient at protein production and accessible for transfection.

How fast do 293t cells grow?

These cell lines are commonly referred to as 293s or 293Ts. On the face of it, 293Ts in particular are very hardy cells. They don’t require specialized media and tend to grow predictably, usually doubling every 24 hours.

What does the 293 mean in HEK293?

human embryonic kidney cells
HEK293 are immortalized human embryonic kidney cells. Originally isolated in the 1970s by Alex Van der Eb, a Dutch biologist, it was postdoc Frank Graham who transformed the cell line with sheared adenovirus 5 (Ad5). The ‘293’ refers to the fact that it was Graham’s 293rd experiment.

How do you maintain a 293t cell?

How many times can you passage 293t cells?

However, the number of passages – not only for HEK293T cells – frequently depends on specifics of the laboratory and/or the person propagting the cells. We propagate HEK293T cells twice a week at split ratio of 1:3 and use bdbiosciences cell culture material. Cultures should be confluent after 3 days.

How fast do 293T cells grow?

What are HEK293T cells used for?

HEK293T cells are a popular derivative of the original HEK293 parent cell line. The HEK293T genome contains the SV40 large T antigen, which enables them to produce recombinant proteins within plasmid vectors containing the SV40 promoter. For this reason, HEK293T is commonly used for retroviral production.

Where do HEK293t cells come from?

Origins of the HEK293 Cell Line HEK293 is a cell line derived from human embryonic kidney cells grown in tissue culture. They are also known, more informally, as HEK cells. This particular line was initiated by the transformation and culturing of normal HEK cells with sheared adenovirus 5 DNA.

Why are HEK293t cells used?

HEK293 are frequently used for heterologous expression of cell membrane receptors and ion channels, and many studies have utilized this cell line to investigate the signalling pathways of G protein-coupled receptors (GPCRs) which are implicated in disease.